| No. | Primer Name | Sequence | Size (mer) | Tm °C | Remarks |
|---|---|---|---|---|---|
| 1 | M13/pUC | 5'AGCGGATAACAATTTCACACAGGA3' | 24 | 70 | reverse primer(-48) |
| 2 | M13/pUC | 5'AACAGCTATGACCATGATTACG3' | 22 | 55 | reverse primer(-21) |
| 3 | M13/pUC | 5'TGTAAAACGACGGCCAGT3' | 18 | 57 | uviversal primer(-20) |
| 4 | M13/pUC | 5'GTTTTCCCAGTCACGACGTTG3' | 21 | 60 | universal primer(-40) |
| 5 | T3 | 5'GCGCGAAATTAACCCTCACTAAAG3' | 24 | 69 | T3 |
| 6 | T7 | 5'TTAATACGACTCACTATAGG3' | 20 | 62 | T7 |
| 7 | SP6 | 5'CCAAGCTATTTAGGTGACAC3' | 20 | 55 | SP6 |
| 8 | SP6A | 5'GCATTTAGGTGACACTATAG3' | 20 | 64 | for pcDNA3 ( reverse) |
| 9 | BGH rev | 5'TAGAAGGCACAGTCGAGG3' | 18 | 55 | for pcDNA3 (reverse) |
| 10 | pET | 5'TAGGGGAATTGTGAGCGGATAAC3' | 23 | 62 | for pET |
| 11 | T7 Terminator | 5'GCTAGTTATTGCTCAGCGGT3' | 20 | 68 | for pET |
| 12 | pGEX5 | 5'GGGCTGGCAAGCCACGTTTGGTG3' | 23 | 79 | 5' |
| 13 | pGEX3 | 5'CCGGGAGCTGCATGTGTCAGAGG3' | 23 | 79 | 3' |
| 14 | POLY-T | 5'TTTTTTTTTTTTTTTTTTTTTTV3' | 23 | 53 | for sequencing down stream poly-T regions |
| 15 | POLY-A | 5'AAAAAAAAAAAAAAAAAAAAAB3' | 23 | 53 | for sequencing down stream poly-A regions |
| 16 | pJET for | 5'CGACTCACTATAGGGAGAGCGGC3' | 23 | 77 | pJET forward |
| 17 | pJET rev | 5'AAGAACATCGATTTTCCATGGCAG3' | 24 | 70 | pJET reverse |
