Isothermal Titration Calorimetry (ITC)

ITC is a method for determining the affinity of biomolecular interactions. An ITC experiment determines all binding and thermodynamic parameters e.g. KD, stoichiometry, ΔG, ΔH, and ΔS. In every binding event, heat is released or absorbed, this heat is measured by a highly sensitive experimental cell and translated into thermodynamic parameters that includes the binding affinity.
An ITC experiment does not require any labeling of the components and allows direct measurement of sub-millimolar to nanomolar binding constants. Furthermore, no immobilization is necessary and there is a free choice of solvent.

Major applications:

Characterization of biomolecular interactions such as:
  • Protein – Protein
  • Protein – Peptide
  • Protein – Small Molecules (Drugs)
  • Protein – Nucleic Acids
  • Metal co-Factor – Protein
Kinetics & thermodynamics of enzyme catalysis

Informational links:



Academic Review Paper

For experiment design and operation of the system, please contact:

Roi Strulovich (tel. 03-6406211). roeistar at