Flow
cytometry
Flow
Cytometry is the measurement of single cells as they flow one by one past laser
beams
with the resulting light scatter and fluorescence emission detected and
analyzed.
Multiple biophysical properties of each individual cell can be
measured at rates of thousands
of cells per second. Cells are identified by
their staining with fluorescent dyes that bind directly
to specific
constituents like DNA, RNA, proteins or ions or with dyes conjugated to
molecules
like antibodies, lectins, or hormones that bind to different cellular
structures.
Cells may also be identified following an infection or tranfection
by marker fluorescent proteins.
Flow Cytometry can detect and analyze multiple
fluorescent staining simultaneously in each
individual cell and can report and
physically separate them by these qualities.
The
Flow Cytometry unit has FACSort with 2 lasers: 488nm and 635nm.
